Evaluation of the effectiveness of RNA silencing in the filamentous fungus Aspergillus niger using a binary vector integrated with Gateway recombination cloning technology

The filamentous fungus Aspergillus niger is widely employed in the production of citric acid and some industrial enzymes such as phytase, glucoamylase, glucose oxidase. Currently, several genetic modification tools have been developed applied to this fungal species. However, RNA silencing technique less used for A. niger. In present study, an binary vector integrated with Gateway recombination cloning technology was evaluated integration vectors rapid generation hairpin structure allowed assessment inhibition target gene expression through mRNA degradation. constructs were successfully transferred into nigervia Agrobacterium tumefaciens. Results showed that effective downregulating DsRed fluorescent reporter stuA regulatory gene. Interestingly, silenced mutants exhibited a significant reduction sporulation. This finding revealed examined represents potential tool functional studies genes